在Python中，可以使用wave模塊來截取一段音頻數據。以下是一個示例代碼，演示如何截取一段音頻數據：

import wave

def extract_audio_segment(input_file, output_file, start_seconds, end_seconds):
    # 打開輸入音頻文件
    with wave.open(input_file, 'rb') as audio_file:
        # 獲取音頻文件的參數
        num_channels = audio_file.getnchannels()
        sample_width = audio_file.getsampwidth()
        frame_rate = audio_file.getframerate()
        num_frames = audio_file.getnframes()

        # 計算截取的起始幀和結束幀
        start_frame = int(start_seconds * frame_rate)
        end_frame = int(end_seconds * frame_rate)
        
        # 限制截取范圍在有效幀數內
        start_frame = min(start_frame, num_frames)
        end_frame = min(end_frame, num_frames)
        
        # 移動文件指針到起始幀
        audio_file.setpos(start_frame)
        
        # 計算截取的幀數
        num_frames_to_extract = end_frame - start_frame
        
        # 打開輸出音頻文件
        with wave.open(output_file, 'wb') as output_audio:
            # 設置輸出音頻文件的參數
            output_audio.setnchannels(num_channels)
            output_audio.setsampwidth(sample_width)
            output_audio.setframerate(frame_rate)
            
            # 從輸入音頻文件中讀取并寫入截取的音頻數據
            output_audio.writeframes(audio_file.readframes(num_frames_to_extract))

使用示例：

input_file = 'input.wav'
output_file = 'output.wav'
start_seconds = 3.5
end_seconds = 8.2

extract_audio_segment(input_file, output_file, start_seconds, end_seconds)

上述代碼將從輸入音頻文件的第3.5秒開始，截取到第8.2秒的音頻數據，并保存到輸出音頻文件中。請確保您已經安裝了wave模塊。